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Wednesday, November 25, 2009

LABORATORY RULES AND REGULATIONS

1. Before accepting borrowed apparatus, inspect carefully each equipment or glassware. Once issued, the Laboratory Technologist will consider all broken equipment as the responsibility of the student.

2. Always sign borrower's slip for equipment borrowed.

3. Equipment must be returned immediately after each laboratory period.

4. Upon the return of the apparatus, the form will be given back to you. If there are some apparatuses not accounted for, the form will remain in file.

5. No laboratory equipment maybe taken out of the laboratory.

6. Provide yourself with a laboratory gown, towel, rag and all the necessary Personal Protective Equipment (PPE).

7. Be aware of the location of fire extinguishers. Put out fires due to inflammable solvent with a wet towel or blanket.

8. Throw away excess inflammable solvent into the sink with large amounts of water.

9. Wrap solid waste with paper, including blood clots, before throwing into the appropriate waste container.

10. Report immediately any damaged or broken equipment to the laboratory technologist.

11. Clean glassware immediately after use.

12. Clean working area thoroughly after each laboratory period.

13. Do not use any instrument in the laboratory without prior permission from your instructor.

14. Do not talk or laugh boisterously inside the laboratory.

15. Do not enter the preparation room without permission.

16. Do not operate the centrifuge machine unless the tubes are properly balanced and are positioned opposite each other.

17. Do not bring volatile solvent like ether or alcohol near an open flame.

18. Eating, drinking and smoking inside the laboratory are strictly prohibited.

19. Do not throw solid substances into the sink to avoid clogging of pipes.

20. Do not splash water on a chemical fire, this would only worsen the spread of the flames.

21. Alert anyone if you smell gas and try to locate the leak.

22. Never pipet by mouth, rubber aspirators are provided for this purpose.

23. Always wash your hands with soap and water before leaving the laboratory.

24. Observe the Universal precaution: “Treat all blood, blood products and other body fluids as potentially infectious, regardless of the knowledge on their HIV - SERO status."


Sunday, November 22, 2009

Assingnments MT 3 -

Deadline would be on Sunday - November 29, 2009


Edsel - colorimetric test for calcium - # 1-10
Fernand0- AAS test for calcium - # 2-10
Robin - Electrophoresis test for calcium # 3-10
Kim - Precautions in calcium test # 4-10
Tiffany - EFP test for calcium # 5 -10
Vixienne - normal values and C.S. for calcium # 6 -10
Jerome - colorimetric method for potassium # 7-10
Paul Arvin - AAS method for potassium # 8 -10
Jenny - Electrohphoresis method for potassium # 9-10
Abigail - EFP test for potassium # 10-10
Kate - precautions for potassium test # 11-10
Johanna - normal values and C.S. # 12-10
Jennie- colorimetric method for sodium # 13-10
Rosette - AAS method for sodium # 13 -10
Mark John - Electrohphoresis method for sodium # 14-10
Lilibeth - EFP test for sodium # 15-10
Justine - precautions for sodium test # 16-10
Jan Kevin - normal values and C.S. # 17-10
Princess - colorimetric method for chloride # 18-10
Aiko - AAS method for chloride # 19 -10
Toni Jerico - Electrohphoresis method for chloride # 20-10
Mark Jadrian - EFP test for chloride # 21-10
John Patrick - precautions for chloride test # 22-10
Jayson - normal values and C.S. of chlordie # 23-10
Noel - precipitation methods for chloride # 24-10
Marjorie - colorimetric method for phosphorus # 25-10
Norilie - AAS method for phosphorus # 26 -10
Lara Mikee - Electrohphoresis method for phosphorus # 27-10
Sheena Marie - EFP test for phosphorus # 28-10
Lea - precautions for phosphorus test # 29-10
Anna Kamille - normal values and C.S. of phosphorus # 30-10
Joseph - colorimetric method for magnesium # 31-10
Chelzylyn - AAS method for magnesium # 32 -10
Kristensen - Electrohphoresis method for magnesium # 33-10
Irish Mirah - precautions for magnesium test # 34-10
student with no designated assignment - normal values and C.S. of magnesium # 35-10


Saturday, November 21, 2009

Clinical Electrolytes - Cations and Anions


Electrolytes are amphoteric substances, meaning they could be positively or negatively charged. The positively charged ions are called cations, and the negatively charged -anions.

Examples given here are those electrolytes which are commonly tested in the clinical laboratory.

Positively charged ions are called CATIONS :

1. Potassium
- intracellular
2. Magnesium - intracellular
3. Calcium - extracellular
4. Sodium - extracellular

Negatively charged ions are called ANIONS :

1. Chloride
- extracellular
2. bicarbonate - extracellular
3. inorganic phosphorus - intracellular

The determination of these electrolytes are very significant in the assessment of several diseases like diabetic ketoacidosis, water-electrolyte status , and many more.


Saturday, November 7, 2009

ATTENTION - CC BLOGGERS

Photo: Courtesy of Red Castro

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