Friday, June 23, 2017

Questions and Answers on Clinical Enzymology (ACP)

1. Discuss the principle of the most common principle of the ACP test.

ACP catalyzes the hydrolysis of Paranitrophenylphosphate, which is colorless, at an acidic pH, to paranitrophenol, which is colored yellow, at 450 to 470 nanometers.

2. Why do you have to utilize a specific wavelength in measuring ACP?

To be able to get the maximum reading of ACP in the sample.

3. What is the ideal specimen for the ACP determination?

Citrated blood is ideal, but for the determination performed in the lab, it is serum.

4. Name sources of errors for ACP test

Hemolyzed serum falsely increases values

Turbid and icteric serum need serum blanking for accuracy

Some reagents are photosensitive, exposure to light would decrease values

Prolonged or shortened incubation time would increase and decrease values respectively

Alkaline pH would decrease values

Altered temperatures could either increase or decrease values

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